Direct detection of DNA methylation during single-molecule, real-time sequencing
نویسندگان
چکیده
منابع مشابه
Real-time fluorescence detection of a single DNA molecule.
The fluorescence detection of polymerase chain reaction (PCR) products using the ABI Model 7700 Sequence Detection System (PE Applied Biosystems, Foster City, CA, USA) allows researchers to rapidly detect and quantify gene sequences without the need for labor-intensive post-PCR processing such as gel electrophoresis and radioactive hybridization (1). This, in conjunction with a built-in, 96-wel...
متن کاملCharacterization of DNA methyltransferase specificities using single-molecule, real-time DNA sequencing
DNA methylation is the most common form of DNA modification in prokaryotic and eukaryotic genomes. We have applied the method of single-molecule, real-time (SMRT®) DNA sequencing that is capable of direct detection of modified bases at single-nucleotide resolution to characterize the specificity of several bacterial DNA methyltransferases (MTases). In addition to previously described SMRT seque...
متن کاملSingle molecule detection of direct, homologous, DNA/DNA pairing.
Using a parallel single molecule magnetic tweezers assay we demonstrate homologous pairing of two double-stranded (ds) DNA molecules in the absence of proteins, divalent metal ions, crowding agents, or free DNA ends. Pairing is accurate and rapid under physiological conditions of temperature and monovalent salt, even at DNA molecule concentrations orders of magnitude below those found in vivo, ...
متن کاملSingle-cell, locus-specific bisulfite sequencing (SLBS) for direct detection of epimutations in DNA methylation patterns
Stochastic epigenetic changes drive biological processes, such as development, aging and disease. Yet, epigenetic information is typically collected from millions of cells, thereby precluding a more precise understanding of cell-to-cell variability and the pathogenic history of epimutations. Here we present a novel procedure for directly detecting epimutations in DNA methylation patterns using ...
متن کاملRapid Sequencing of DNA Based On Single Molecule Detection
Sequencing the human genome is a major undertaking considering the large number of nucleotides present in the genome and the slow methods currently available to perform the task. We have recently reported on a scheme to sequence DNA rapidly using a non-gel based technique. The concept is based upon the incorporation of fluorescently labeled nucleotides into a strand of DNA, isolation and manipu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Methods
سال: 2010
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.1459